proteome profiler human chemokine array kit  (R&D Systems)

Journal: Aging Cell

Article Title: Targeting Hyperoxia‐Induced Cellular Senescence in Developing Human Airway Cells: Senomorphics Versus Senolytics Versus Antioxidants

doi: 10.1111/acel.70538

Figure Lengend Snippet: Hyperoxia‐induced senescent fASM secrete higher levels of SASP. Cells cultured till day 7 in normoxic (21% O 2 ) or hyperoxic (50% O 2 ) environment were incubated with fresh growth media in normoxia for 24 h, and the supernatants were collected. Samples were then analyzed for SASP secretion by Eve Technologies Corporation (Calgary, Alberta, Canada) using Luminex xMAP and the Human Cytokine/Chemokine 96‐Plex Discovery Assay Array (HD96) panel. (A) Out of the 96 proteins, 80 were detected and visualized using a heatmap. (B) A detailed analysis of each detected marker is shown as fold‐change normalized to control. For statistical analysis, 2‐way ANOVA with a two‐stage linear step‐up procedure of Benjamini, Krieger and Yekutieli test, with individual variances computed for each comparison was applied. False discovery rate < 0.05 was considered significant. Data are presented as box plot of n = 7 cell lines per group.

Article Snippet: Supernatants collected at day 8 from normoxia and hyperoxia‐exposed fASM were analyzed for SASP secretion by Eve Technologies Corporation (Calgary, Alberta, Canada) using the Human Cytokine/Chemokine 96‐Plex Discovery Assay Array (HD96) panel.

Techniques: Cell Culture, Incubation, Luminex, Marker, Control, Comparison

Journal: Frontiers in Immunology

Article Title: B-type natriuretic peptide attenuates TLR-induced cytokine and chemokine secretion in monocyte-derived Langerhans cells

doi: 10.3389/fimmu.2026.1791284

Figure Lengend Snippet: BNP treatment increased moLCs migration toward CCL21 and reduced chemokine production in activated moLCs. Monocytes were cultured in the presence of GMCSF, TNF-α, and TGF-β for 5 days supplemented with IL-4 for the first 48 hrs to differentiate moLCs, and treated with 10 nM BNP throughout the entire differentiation process. On day 4, moLCs were activated with CL075 and poly(I:C) and a combination of both for 24 hrs. (A) In Transwell migration assay, 1*10 6 cells were applied in the upper well of the Transwell plate, and in the bottom of the plate, it contains RPMI supplemented with CCL19 and CCL21. Migration of the cells was measured with flow cytometry. Data are presented as individual values with mean ± SEM. Statistical significance was determined by Two-way ANOVA followed by Tukey’s multiple comparison test. Normality of residuals was confirmed by QQ plot analysis. n=8 biological replicates per group (B) A chemokine array was performed using supernatants from BNP treated and TLR activated cells. Symbols with different colors represent individual donors. Data is presented as Mean ± SEM. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analysis. n=2 biological replicates per group *P<0.05, **P<0.01, *** P<0.001, **** P< 0.0001. BNP, B-type natriuretic peptide; CL075; TLR7/8 agonist, thiazoquinoline compound; CTRL, Control; moLC, monocyte-derived Langerhans cells; Poly(I:C), TLR3 agonist, polyinosinic:polycytidylic acid.

Article Snippet: Proteome Profiler Human Chemokine Array Kit (R&D Systems) was performed according to the manufacturer’s instructions.

Techniques: Migration, Cell Culture, Transwell Migration Assay, Flow Cytometry, Comparison, Control, Derivative Assay